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Scheme for generating the humanized NSG mice. MNCs isolated from hUCB were enriched using a <t>RosetteSep</t> kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.
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Scheme for generating the humanized NSG mice. MNCs isolated from hUCB were enriched using a RosetteSep kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.

Journal: Blood research

Article Title: Humanizing NOD/SCID/IL-2Rγnull (NSG) mice using busulfan and retro-orbital injection of umbilical cord blood-derived CD34 + cells

doi: 10.5045/br.2016.51.1.31

Figure Lengend Snippet: Scheme for generating the humanized NSG mice. MNCs isolated from hUCB were enriched using a RosetteSep kit and human CD34 MicroBead Kit. NSG mice were conditioned by busulfan and CD34 + cells were injected via retro-orbital sinus.

Article Snippet: Mononuclear cells (MNCs) were enriched using a RosetteSep Human Progenitor Enrichment kit (StemCell Technologies, Canada) and isolated from hUCB using Ficoll-Paque PREMIUM (GE Healthcare Bio-Sciences AB, Sweden) density gradient centrifugation.

Techniques: Isolation, Injection